A novel patatin-like gene stimulated by drought stress encodes a galactolipid acyl hydrolase
نویسندگان
چکیده
منابع مشابه
A patatin-like protein with galactolipase activity is induced by drought stress in Vigna unguiculata leaves.
This paper reports the cloning of a cDNA (Vupat1) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were examined in Northern-blot analyses. Expression of Vupat1 is stimulated by drought stress, especially in the drought-sen...
متن کاملCharacterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector.
Patatin is a family of glycoproteins that accounts for 30-40% of the total soluble protein in potato (Solanum tuberosum) tubers. This protein has been reported not only to serve as a storage protein, but also to exhibit enzymic activity. By using a baculovirus system to express protein from the patatin cDNA clone pGM01, it was unambiguously shown that the patatin coded by this DNA has lipid acy...
متن کاملorf4 of the Bacillus cereus sigB gene cluster encodes a general stress-inducible Dps-like bacterioferritin.
The function of orf4 in the sigB cluster in Bacillus cereus ATCC 14579 remains to be explored. Amino-acid sequence analysis has revealed that Orf4 is homologous with bacterioferritins and Dps. In this study, we generated an orf4-null mutant and produced recombinant protein rOrf4 to establish the role of orf4. In vitro, the purified rOrf4 was found to exist in two distinct forms, a dimeric form ...
متن کاملStructural organization of the rat acyl-peptide hydrolase gene.
The entire nucleotide sequence of the rat acyl-peptide hydrolase gene including its 5* and 3' flanking region is presented here. Previously, the isolation of the rat acyl-peptide hydrolase (1) and its cDNA (2) were reported. By sequential screening with the cDNA and a 5 cDNA fragment, two overlapping genomic clones (APHE5 and APHH6) were isolated from two Charon 4A rat genomic libraries. From t...
متن کاملCytomegalovirus immediate early gene UL37 encodes a novel MHC-like protein.
The cytomegalovirus (CMV) genome encodes four clusters of genes expressed immediately after infection--i.e.: UL36-38, UL122-123, TRS1-IRS1, and US3. The general function of these genes is associated with inhibition of cellular mechanisms of antiviral response. Although several biological processes have been mapped onto specific gene products, the knowledge of the molecular mechanism of their ac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2001
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(01)02194-9